Function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome coronavirus.
Identifieur interne : 004827 ( Main/Exploration ); précédent : 004826; suivant : 004828Function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome coronavirus.
Auteurs : Zhinan Chen [République populaire de Chine] ; Li Mi ; Jing Xu ; Jiyun Yu ; Xianhui Wang ; Jianli Jiang ; Jinliang Xing ; Peng Shang ; Airong Qian ; Yu Li ; Peter X. Shaw ; Jianwei Wang ; Shumin Duan ; Jin Ding ; Chunmei Fan ; Yang Zhang ; Yong Yang ; Xiaoling Yu ; Qiang Feng ; Biehu Li ; Xiying Yao ; Zheng Zhang ; Ling Li ; Xiaoping Xue ; Ping ZhuSource :
- The Journal of infectious diseases [ 0022-1899 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- pathogénicité : Virus du SRAS.
- physiologie : Antigènes CD, Cyclophiline A, Effet cytopathogène viral, Protéines nucléocapside, Protéines virales structurales.
- Expression des gènes, Humains, Liaison aux protéines, Lignée cellulaire.
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : Antigens, CD, Cyclophilin A, Nucleocapsid Proteins, Viral Structural Proteins.
- chemical : Basigin.
- pathogenicity : SARS Virus.
- physiology : Cytopathogenic Effect, Viral.
- Cell Line, Gene Expression, Humans, Protein Binding.
Abstract
To identify the function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome (SARS) coronavirus (CoV), we analyzed the protein-protein interaction among HAb18G/CD147, cyclophilin A (CyPA), and SARS-CoV structural proteins by coimmunoprecipitation and surface plasmon resonance analysis. Although none of the SARS-CoV proteins was found to be directly bound to HAb18G/CD147, the nucleocapsid (N) protein of SARS-CoV was bound to CyPA, which interacted with HAb18G/CD147. Further research showed that HAb18G/CD147, a transmembrane molecule, was highly expressed on 293 cells and that CyPA was integrated with SARS-CoV. HAb18G/CD147-antagonistic peptide (AP)-9, an AP of HAb18G/CD147, had a high rate of binding to 293 cells and an inhibitory effect on SARS-CoV. These results show that HAb18G/CD147, mediated by CyPA bound to SARS-CoV N protein, plays a functional role in facilitating invasion of host cells by SARS-CoV. Our findings provide some evidence for the cytologic mechanism of invasion by SARS-CoV and provide a molecular basis for screening anti-SARS drugs.
DOI: 10.1086/427811
PubMed: 15688292
Affiliations:
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Shaw</name></author><author><name sortKey="Wang, Jianwei" sort="Wang, Jianwei" uniqKey="Wang J" first="Jianwei" last="Wang">Jianwei Wang</name></author><author><name sortKey="Duan, Shumin" sort="Duan, Shumin" uniqKey="Duan S" first="Shumin" last="Duan">Shumin Duan</name></author><author><name sortKey="Ding, Jin" sort="Ding, Jin" uniqKey="Ding J" first="Jin" last="Ding">Jin Ding</name></author><author><name sortKey="Fan, Chunmei" sort="Fan, Chunmei" uniqKey="Fan C" first="Chunmei" last="Fan">Chunmei Fan</name></author><author><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name></author><author><name sortKey="Yang, Yong" sort="Yang, Yong" uniqKey="Yang Y" first="Yong" last="Yang">Yong Yang</name></author><author><name sortKey="Yu, Xiaoling" sort="Yu, Xiaoling" uniqKey="Yu X" first="Xiaoling" last="Yu">Xiaoling Yu</name></author><author><name sortKey="Feng, Qiang" sort="Feng, Qiang" uniqKey="Feng Q" first="Qiang" last="Feng">Qiang Feng</name></author><author><name sortKey="Li, Biehu" sort="Li, Biehu" uniqKey="Li B" first="Biehu" last="Li">Biehu Li</name></author><author><name sortKey="Yao, Xiying" sort="Yao, Xiying" uniqKey="Yao X" first="Xiying" last="Yao">Xiying Yao</name></author><author><name sortKey="Zhang, Zheng" sort="Zhang, Zheng" uniqKey="Zhang Z" first="Zheng" last="Zhang">Zheng Zhang</name></author><author><name sortKey="Li, Ling" sort="Li, Ling" uniqKey="Li L" first="Ling" last="Li">Ling Li</name></author><author><name sortKey="Xue, Xiaoping" sort="Xue, Xiaoping" uniqKey="Xue X" first="Xiaoping" last="Xue">Xiaoping Xue</name></author><author><name sortKey="Zhu, Ping" sort="Zhu, Ping" uniqKey="Zhu P" first="Ping" last="Zhu">Ping Zhu</name></author></analytic><series><title level="j">The Journal of infectious diseases</title><idno type="ISSN">0022-1899</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, CD (physiology)</term><term>Basigin</term><term>Cell Line</term><term>Cyclophilin A (physiology)</term><term>Cytopathogenic Effect, Viral (physiology)</term><term>Gene Expression</term><term>Humans</term><term>Nucleocapsid Proteins (physiology)</term><term>Protein Binding</term><term>SARS Virus (pathogenicity)</term><term>Viral Structural Proteins (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigènes CD (physiologie)</term><term>Cyclophiline A (physiologie)</term><term>Effet cytopathogène viral (physiologie)</term><term>Expression des gènes</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Protéines nucléocapside (physiologie)</term><term>Protéines virales structurales (physiologie)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Antigens, CD</term><term>Cyclophilin A</term><term>Nucleocapsid Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Basigin</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Antigènes CD</term><term>Cyclophiline A</term><term>Effet cytopathogène viral</term><term>Protéines nucléocapside</term><term>Protéines virales structurales</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cytopathogenic Effect, Viral</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Gene Expression</term><term>Humans</term><term>Protein Binding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Expression des gènes</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To identify the function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome (SARS) coronavirus (CoV), we analyzed the protein-protein interaction among HAb18G/CD147, cyclophilin A (CyPA), and SARS-CoV structural proteins by coimmunoprecipitation and surface plasmon resonance analysis. Although none of the SARS-CoV proteins was found to be directly bound to HAb18G/CD147, the nucleocapsid (N) protein of SARS-CoV was bound to CyPA, which interacted with HAb18G/CD147. Further research showed that HAb18G/CD147, a transmembrane molecule, was highly expressed on 293 cells and that CyPA was integrated with SARS-CoV. HAb18G/CD147-antagonistic peptide (AP)-9, an AP of HAb18G/CD147, had a high rate of binding to 293 cells and an inhibitory effect on SARS-CoV. These results show that HAb18G/CD147, mediated by CyPA bound to SARS-CoV N protein, plays a functional role in facilitating invasion of host cells by SARS-CoV. Our findings provide some evidence for the cytologic mechanism of invasion by SARS-CoV and provide a molecular basis for screening anti-SARS drugs.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Ding, Jin" sort="Ding, Jin" uniqKey="Ding J" first="Jin" last="Ding">Jin Ding</name><name sortKey="Duan, Shumin" sort="Duan, Shumin" uniqKey="Duan S" first="Shumin" last="Duan">Shumin Duan</name><name sortKey="Fan, Chunmei" sort="Fan, Chunmei" uniqKey="Fan C" first="Chunmei" last="Fan">Chunmei Fan</name><name sortKey="Feng, Qiang" sort="Feng, Qiang" uniqKey="Feng Q" first="Qiang" last="Feng">Qiang Feng</name><name sortKey="Jiang, Jianli" sort="Jiang, Jianli" uniqKey="Jiang J" first="Jianli" last="Jiang">Jianli Jiang</name><name sortKey="Li, Biehu" sort="Li, Biehu" uniqKey="Li B" first="Biehu" last="Li">Biehu Li</name><name sortKey="Li, Ling" sort="Li, Ling" uniqKey="Li L" first="Ling" last="Li">Ling Li</name><name sortKey="Li, Yu" sort="Li, Yu" uniqKey="Li Y" first="Yu" last="Li">Yu Li</name><name sortKey="Mi, Li" sort="Mi, Li" uniqKey="Mi L" first="Li" last="Mi">Li Mi</name><name sortKey="Qian, Airong" sort="Qian, Airong" uniqKey="Qian A" first="Airong" last="Qian">Airong Qian</name><name sortKey="Shang, Peng" sort="Shang, Peng" uniqKey="Shang P" first="Peng" last="Shang">Peng Shang</name><name sortKey="Shaw, Peter X" sort="Shaw, Peter X" uniqKey="Shaw P" first="Peter X" last="Shaw">Peter X. Shaw</name><name sortKey="Wang, Jianwei" sort="Wang, Jianwei" uniqKey="Wang J" first="Jianwei" last="Wang">Jianwei Wang</name><name sortKey="Wang, Xianhui" sort="Wang, Xianhui" uniqKey="Wang X" first="Xianhui" last="Wang">Xianhui Wang</name><name sortKey="Xing, Jinliang" sort="Xing, Jinliang" uniqKey="Xing J" first="Jinliang" last="Xing">Jinliang Xing</name><name sortKey="Xu, Jing" sort="Xu, Jing" uniqKey="Xu J" first="Jing" last="Xu">Jing Xu</name><name sortKey="Xue, Xiaoping" sort="Xue, Xiaoping" uniqKey="Xue X" first="Xiaoping" last="Xue">Xiaoping Xue</name><name sortKey="Yang, Yong" sort="Yang, Yong" uniqKey="Yang Y" first="Yong" last="Yang">Yong Yang</name><name sortKey="Yao, Xiying" sort="Yao, Xiying" uniqKey="Yao X" first="Xiying" last="Yao">Xiying Yao</name><name sortKey="Yu, Jiyun" sort="Yu, Jiyun" uniqKey="Yu J" first="Jiyun" last="Yu">Jiyun Yu</name><name sortKey="Yu, Xiaoling" sort="Yu, Xiaoling" uniqKey="Yu X" first="Xiaoling" last="Yu">Xiaoling Yu</name><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name><name sortKey="Zhang, Zheng" sort="Zhang, Zheng" uniqKey="Zhang Z" first="Zheng" last="Zhang">Zheng Zhang</name><name sortKey="Zhu, Ping" sort="Zhu, Ping" uniqKey="Zhu P" first="Ping" last="Zhu">Ping Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Chen, Zhinan" sort="Chen, Zhinan" uniqKey="Chen Z" first="Zhinan" last="Chen">Zhinan Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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